Human Anosmin-1(KAL1) ELISA kit
Product Details
| Target Name |
Kallmann syndrome 1 sequence |
| Alternative Names |
Adhesion molecule-like X-linked ELISA Kit; ADMLX ELISA Kit; Anosmin-1 ELISA Kit; HHA ELISA Kit; KAL ELISA Kit; KAL1 ELISA Kit; KALIG 1 ELISA Kit; KALIG1 ELISA Kit; Kallmann syndrome 1 sequence (anosmin 1) ELISA Kit; Kallmann syndrome 1 sequence ELISA Kit; Kallmann syndrome interval gene 1 ELISA Kit; Kallmann syndrome protein ELISA Kit; KALM ELISA Kit; KALM_HUMAN ELISA Kit; KMS ELISA Kit; OTTHUMP00000022873 ELISA Kit |
| Abbreviation |
KAL1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
125 pg/mL-8000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human KAL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
99 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
89 |
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| Range % |
85-94 |
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| Recovery |
| The recovery of human KAL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
94-104 |
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| EDTA plasma (n=4) |
96 |
91-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 8000 |
2.487 |
2.466 |
2.477 |
2.375 |
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| 4000 |
2.191 |
2.180 |
2.186 |
2.084 |
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| 2000 |
1.828 |
1.856 |
1.842 |
1.740 |
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| 1000 |
1.355 |
1.397 |
1.376 |
1.274 |
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| 500 |
0.962 |
0.991 |
0.977 |
0.875 |
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| 250 |
0.554 |
0.594 |
0.574 |
0.472 |
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| 125 |
0.298 |
0.281 |
0.290 |
0.188 |
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| 0 |
0.101 |
0.103 |
0.102 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
