Human Anterior gradient protein 3 homolog(AGR3) ELISA kit
Product Details
| Target Name |
anterior gradient homolog 3 (Xenopus laevis) |
| Alternative Names |
AG-3 ELISA Kit; AG3 ELISA Kit; Agr3 ELISA Kit; AGR3_HUMAN ELISA Kit; Anterior gradient 3; xenopus; homolog of ELISA Kit; Anterior gradient homolog 3 ELISA Kit; Anterior gradient homolog 3 (Xenopus laevis) ELISA Kit; anterior gradient protein 3 ELISA Kit; Anterior gradient protein 3 homolog ELISA Kit; BCMP11 ELISA Kit; Breast cancer membrane protein 11 ELISA Kit; Gm888 ELISA Kit; hAG 3 ELISA Kit; hAG-3 ELISA Kit; HAG3 ELISA Kit; OTTHUMP00000158512 ELISA Kit; PDIA18 ELISA Kit; Protein disulfide isomerase family A member 18 ELISA Kit |
| Abbreviation |
AGR3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
85 |
|
| Range % |
80-89 |
|
| 1:2 |
Average % |
91 |
|
| Range % |
86-95 |
|
| 1:4 |
Average % |
94 |
|
| Range % |
90-98 |
|
| 1:8 |
Average % |
97 |
|
| Range % |
92-101 |
|
|
| Recovery |
| The recovery of human AGR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-95 |
|
| EDTA plasma (n=4) |
94 |
89-99 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.713 |
2.662 |
2.688 |
2.515 |
|
| 5 |
2.376 |
2.431 |
2.404 |
2.231 |
|
| 2.5 |
1.934 |
2.001 |
1.968 |
1.795 |
|
| 1.25 |
1.509 |
1.582 |
1.546 |
1.373 |
|
| 0.625 |
0.974 |
1.020 |
0.997 |
0.824 |
|
| 0.312 |
0.564 |
0.559 |
0.562 |
0.389 |
|
| 0.156 |
0.296 |
0.279 |
0.288 |
0.115 |
|
| 0 |
0.178 |
0.167 |
0.173 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
