Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit
Product Details
| Description |
This Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody, ACCPA) ELISA Kit is designed to qualitatively detect the anti-CCP antibody in the serum. It employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CCP antigen. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Any antibodies specific for CCP present in the samples will bind to the pre-coated CCP antigen. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human anti-CCP antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. This assay has high sensitivity and excellent specificity for detection of human anti-CCP antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product in... Read more |
| Target Name |
anti-cyclic citrullinated peptide antibody (anti-CCP antibody) |
| Abbreviation |
ACCPA |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum |
| Detection Range |
Request Information |
| Sensitivity |
Request Information |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Autoimmunity |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15>
| |
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<15>
| |
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
| Test parameter |
specification |
test result |
|
| Positive control |
>1 |
2.078 |
|
| Negative control |
<0>
| 0.075 |
|
| Positive rate |
10，Positive |
100% |
|
| Negative rate |
10，Negative |
100% |
|
|
| Materials provided |
- A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen.
- Negative Control (1 x 0.8 ml)
- Positive Control (1 x 0.8 ml)
- HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration.
- Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
