Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit
Product Details
| Target Name |
anti-mutated citrullinated vimentin (MCV) antibody |
| Abbreviation |
MCV Ab |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
Request Information |
| Sensitivity |
Request Information |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
qualitative |
| Measurement |
Indirect |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-MCV antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
88 |
|
| Range % |
80-96 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
91-99 |
|
| 1:4 |
Average % |
89 |
|
| Range % |
85-96 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
86-101 |
|
|
| Recovery |
| The recovery of human anti-MCV antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-101 |
|
| EDTA plasma (n=4) |
94 |
90-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| U/ml |
OD1 |
OD2 |
Average |
|
|
| 400 |
1.257 |
1.290 |
1.274 |
|
|
| 100 |
0.636 |
0.627 |
0.632 |
|
|
| 25 |
0.184 |
0.197 |
0.191 |
|
|
| 10 |
0.121 |
0.112 |
0.117 |
|
|
| 5 |
0.051 |
0.048 |
0.050 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
