Human Apelin receptor(APLNR) ELISA kit
Product Details
| Target Name |
apelin receptor |
| Alternative Names |
AGTR L1 ELISA Kit; AGTRL 1 ELISA Kit; AGTRL1 ELISA Kit; Angiotensin II receptor like 1 ELISA Kit; Angiotensin receptor like 1 ELISA Kit; Angiotensin receptor-like 1 ELISA Kit; Apelin receptor ELISA Kit; APJ (apelin) receptor ELISA Kit; APJ ELISA Kit; APJ receptor ELISA Kit; APJ_HUMAN ELISA Kit; APJR ELISA Kit; APLNR ELISA Kit; B78 ELISA Kit; FLJ90771 ELISA Kit; G protein coupled receptor APJ ELISA Kit; G-protein coupled receptor APJ ELISA Kit; G-protein coupled receptor HG11 ELISA Kit; GPCR34 ELISA Kit; HG 11 ELISA Kit; HG11 ELISA Kit; HG11 orphan receptor ELISA Kit; MGC45246 ELISA Kit; MSR ELISA Kit; msr/apj ELISA Kit |
| Abbreviation |
APLNR |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
23.5 pg/mL-1500 pg/mL |
| Sensitivity |
5.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human APLNR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
94-103 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-105 |
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| Recovery |
| The recovery of human APLNR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
87-94 |
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| EDTA plasma (n=4) |
96 |
94-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1500 |
2.312 |
2.189 |
2.251 |
2.081 |
|
| 750 |
1.494 |
1.455 |
1.475 |
1.305 |
|
| 375 |
1.026 |
0.994 |
1.010 |
0.840 |
|
| 187.5 |
0.655 |
0.688 |
0.672 |
0.502 |
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| 94 |
0.448 |
0.442 |
0.445 |
0.275 |
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| 47 |
0.303 |
0.292 |
0.298 |
0.128 |
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| 23.5 |
0.241 |
0.232 |
0.237 |
0.067 |
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| 0 |
0.163 |
0.177 |
0.170 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
