Human apolipoprotein E (Apo-E) ELISA Kit
Product Details
| Target Name |
apolipoprotein E |
| Alternative Names |
AD2 ELISA Kit; Apo-E ELISA Kit; APOE ELISA Kit; APOE_HUMAN ELISA Kit; APOEA ELISA Kit; Apolipoprotein E ELISA Kit; Apolipoprotein E3 ELISA Kit; ApolipoproteinE ELISA Kit; Apoprotein ELISA Kit; LDLCQ5 ELISA Kit; LPG ELISA Kit |
| Abbreviation |
APOE |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
15.62 ng/mL-1000 ng/mL |
| Sensitivity |
3.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
91 |
|
| Range % |
86-95 |
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| 1:200 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:400 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:800 |
Average % |
97 |
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| Range % |
91-103 |
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| Recovery |
| The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
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| 1000 |
0.088 |
0.090 |
0.089 |
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| 500 |
0.135 |
0.142 |
0.139 |
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| 250 |
0.227 |
0.237 |
0.232 |
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| 125 |
0.324 |
0.341 |
0.333 |
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| 62.5 |
0.583 |
0.598 |
0.591 |
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| 31.25 |
0.847 |
0.864 |
0.856 |
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| 15.62 |
1.228 |
1.235 |
1.232 |
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| 0 |
2.155 |
2.199 |
2.177 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
