Human Apolipoprotein M(APOM) ELISA kit
Product Details
| Description |
The product CSB-EL001947HU is a ready-to-use microwell, strip plate ELISA Kit for quantitatively measuring the amount of the Apolipoprotein M (ApoM) in human serum, urine, or tissue homogenates. This assay kit was designed and optimized for signal transduction research use in humans. It has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which ApoM in the samples or standards are sandwiched between pre-coated ApoM antibody and Biotin-conjugated ApoM antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoM bound in the initial step. The color development is stopped upon adding the stop solution, and the intensi... Read more |
| Target Name |
apolipoprotein M |
| Alternative Names |
Apo M ELISA Kit; Apo-M ELISA Kit; Apolipoprotein M ELISA Kit; ApoM ELISA Kit; APOM_HUMAN ELISA Kit; G3A ELISA Kit; HSPC336 ELISA Kit; MGC22400 ELISA Kit; NG20 ELISA Kit; NG20 like protein ELISA Kit; Protein G3a ELISA Kit |
| Abbreviation |
APOM |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
84 |
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| Range % |
80-96 |
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| 1:2000 |
Average % |
95 |
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| Range % |
92-102 |
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| 1:4000 |
Average % |
98 |
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| Range % |
92-110 |
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| 1:8000 |
Average % |
95 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of human APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-98 |
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| EDTA plasma (n=4) |
96 |
92-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.566 |
2.547 |
2.557 |
2.464 |
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| 50 |
2.011 |
1.995 |
2.003 |
1.910 |
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| 25 |
1.409 |
1.394 |
1.402 |
1.309 |
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| 12.5 |
0.867 |
0.896 |
0.882 |
0.789 |
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| 6.25 |
0.524 |
0.502 |
0.513 |
0.420 |
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| 3.12 |
0.345 |
0.364 |
0.355 |
0.262 |
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| 1.56 |
0.213 |
0.217 |
0.215 |
0.122 |
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| 0 |
0.094 |
0.091 |
0.093 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ApoM antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ApoM antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
