Human Apoptosis regulator BAX ELISA Kit
Product Details
| Target Name |
BCL2-associated X protein |
| Alternative Names |
Apoptosis regulator BAX ELISA Kit; BAX ELISA Kit; Bax-protein ELISA Kit; BAX_HUMAN ELISA Kit; BAXA ELISA Kit; Baxdelta2G9 ELISA Kit; Baxdelta2G9omega ELISA Kit; Baxdelta2omega ELISA Kit; Bcl-2-like protein 4 ELISA Kit; BCL2 associated X protein ELISA Kit; BCL2 associated X protein omega ELISA Kit; BCL2 associated X protein transcript variant delta2 ELISA Kit; Bcl2-L-4 ELISA Kit; BCL2L4 ELISA Kit; membrane isoform alpha ELISA Kit |
| Abbreviation |
BAX |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.25 ng/mL-80 ng/mL |
| Sensitivity |
0.312 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
87-101 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-97 |
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| Recovery |
| The recovery of human BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
96-109 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.305 |
2.215 |
2.260 |
2.108 |
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| 40 |
1.675 |
1.765 |
1.720 |
1.568 |
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| 20 |
1.164 |
1.172 |
1.168 |
1.016 |
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| 10 |
0.795 |
0.809 |
0.802 |
0.650 |
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| 5 |
0.453 |
0.447 |
0.450 |
0.298 |
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| 2.5 |
0.322 |
0.336 |
0.329 |
0.177 |
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| 1.25 |
0.225 |
0.219 |
0.222 |
0.070 |
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| 0 |
0.152 |
0.151 |
0.152 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
