Human Arginase 2(ARG2) ELISA Kit
Product Details
| Target Name |
arginase, type II |
| Alternative Names |
ARG2 ELISA Kit; ARGI2_HUMAN ELISA Kit; Arginase II mitochondrial ELISA Kit; Arginase type II ELISA Kit; Arginase-2 ELISA Kit; arginase-2, mitochondrial ELISA Kit; Kidney arginase ELISA Kit; Kidney type arginase ELISA Kit; Kidney-type arginase ELISA Kit; L arginine amidinohydrolase ELISA Kit; L arginine ureahydrolase ELISA Kit; mitochondrial ELISA Kit; Non hepatic arginase ELISA Kit; Non-hepatic arginase ELISA Kit; Nonhepatic arginase ELISA Kit; Type II arginase ELISA Kit |
| Abbreviation |
ARG2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF) |
| Detection Range |
0.47 mU/mL-30 mU/mL |
| Sensitivity |
0.12 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
83-91 |
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| 1:2 |
Average % |
104 |
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| Range % |
101-107 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-99 |
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| 1:8 |
Average % |
85 |
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| Range % |
82-89 |
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| Recovery |
| The recovery of human ARG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
91-98 |
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| EDTA plasma (n=4) |
90 |
86-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 30 |
2.327 |
2.298 |
2.313 |
2.146 |
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| 15 |
1.820 |
1.801 |
1.811 |
1.644 |
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| 7.5 |
1.393 |
1.378 |
1.386 |
1.219 |
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| 3.75 |
0.979 |
0.998 |
0.989 |
0.822 |
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| 1.87 |
0.564 |
0.610 |
0.587 |
0.420 |
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| 0.94 |
0.396 |
0.376 |
0.386 |
0.219 |
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| 0.47 |
0.273 |
0.295 |
0.284 |
0.117 |
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| 0 |
0.166 |
0.168 |
0.167 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
