Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit
Product Details
| Target Name |
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) |
| Alternative Names |
AT5G1_HUMAN ELISA Kit; ATP synthase c subunit ELISA Kit; ATP synthase F(0) complex subunit C1; mitochondrial ELISA Kit; ATP synthase F(0) complex subunit C2; mitochondrial ELISA Kit; ATP synthase F(0) complex subunit C3; mitochondrial ELISA Kit; ATP synthase lipid binding protein mitochondrial ELISA Kit; ATP synthase lipid-binding protein ELISA Kit; ATP synthase proteolipid P1 ELISA Kit; ATP synthase proteolipid P2 ELISA Kit; ATP synthase proteolipid P3 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c (subunit 9); isoform 1 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c (subunit 9); isoform 2 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 1 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 2 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 3 ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C1 (subunit 9) ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C2 (subunit 9) ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C3 (subunit 9) ELISA Kit; ATP synthase; mitochondrial; C subunit-3 ELISA Kit; ATP5A ELISA Kit; ATP5G ELISA Kit; ATP5G1 ELISA Kit; ATPase protein 9 ELISA Kit; ATPase subunit 9 ELISA Kit; ATPase subunit c ELISA Kit; mitochondrial ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 1 ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 3 ELISA Kit; mitochondrial ATP synthase; subunit C (subunit 9); isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 1 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 3 ELISA Kit; P3 ELISA Kit |
| Abbreviation |
ATP5G1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
23.5 pg/mL-1500 pg/mL |
| Sensitivity |
5.86 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-92 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-99 |
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| Recovery |
| The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
91-97 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1500 |
2.637 |
2.612 |
2.625 |
2.480 |
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| 750 |
2.215 |
2.118 |
2.167 |
2.022 |
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| 375 |
1.697 |
1.683 |
1.690 |
1.545 |
|
| 187.5 |
1.107 |
1.118 |
1.113 |
0.968 |
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| 94 |
0.611 |
0.621 |
0.616 |
0.471 |
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| 47 |
0.365 |
0.372 |
0.369 |
0.224 |
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| 23.5 |
0.242 |
0.231 |
0.237 |
0.092 |
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| 0 |
0.146 |
0.144 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
