Human atrial natriuretic peptide (ANP) ELISA kit
Product Details
| Target Name |
natriuretic peptide precursor A |
| Alternative Names |
ANF ELISA Kit; ANF_HUMAN ELISA Kit; ANP ELISA Kit; ATFB6 ELISA Kit; Atrial natriuretic factor ELISA Kit; Atrial natriuretic peptide ELISA Kit; Atriopeptin ELISA Kit; Cardiodilatin ELISA Kit; Cardiodilatin related peptide ELISA Kit; Cardiodilatin-related peptide ELISA Kit; Cardionatrin ELISA Kit; CDD ANF ELISA Kit; CDD ELISA Kit; CDD-ANF ELISA Kit; CDP ELISA Kit; Natriuretic peptide A ELISA Kit; Natriuretic peptide precursor A ELISA Kit; NPPA ELISA Kit; PND ELISA Kit; Prepronatriodilatin ELISA Kit |
| Abbreviation |
NPPA |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, cell culture supernates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-98 |
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| 1:8 |
Average % |
101 |
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| Range % |
95-108 |
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| Recovery |
| The recovery of human ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
87-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.932 |
2.789 |
2.861 |
2.771 |
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| 1000 |
2.178 |
2.132 |
2.155 |
2.065 |
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| 500 |
1.566 |
1.555 |
1.561 |
1.471 |
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| 250 |
0.789 |
0.778 |
0.784 |
0.694 |
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| 125 |
0.462 |
0.456 |
0.459 |
0.369 |
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| 62.5 |
0.278 |
0.267 |
0.273 |
0.183 |
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| 31.25 |
0.226 |
0.218 |
0.222 |
0.132 |
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| 0 |
0.093 |
0.087 |
0.090 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
