Human B- and T-lymphocyte attenuator(BTLA) ELISA kit
Product Details
| Target Name |
B and T lymphocyte associated |
| Alternative Names |
B and T lymphocyte associated protein ELISA Kit; B and T lymphocyte attenuator ELISA Kit; B and T lymphocyte associated ELISA Kit; B- and T-lymphocyte attenuator ELISA Kit; B- and T-lymphocyte-associated protein ELISA Kit; BTLA ELISA Kit; BTLA_HUMAN ELISA Kit; BTLA1 ELISA Kit; CD272 ELISA Kit; CD272 antigen ELISA Kit; FLJ16065 ELISA Kit; MGC129743 ELISA Kit |
| Abbreviation |
BTLA |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.45 ng/mL-30 ng/mL |
| Sensitivity |
0.11 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BTLA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
108 |
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| Range % |
102-115 |
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| 1:8 |
Average % |
95 |
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| Range % |
87-100 |
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| Recovery |
| The recovery of human BTLA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-102 |
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| EDTA plasma (n=4) |
99 |
96-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 30 |
2.167 |
2.247 |
2.207 |
2.107 |
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| 15 |
1.532 |
1.456 |
1.494 |
1.394 |
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| 7.5 |
0.928 |
0.954 |
0.941 |
0.841 |
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| 3.75 |
0.589 |
0.579 |
0.584 |
0.484 |
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| 1.8 |
0.387 |
0.401 |
0.394 |
0.294 |
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| 0.9 |
0.286 |
0.274 |
0.280 |
0.180 |
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| 0.45 |
0.193 |
0.199 |
0.196 |
0.096 |
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| 0 |
0.101 |
0.099 |
0.100 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
