Human B cell activation factor，BAFF ELISA Kit
Product Details
| Target Name |
tumor necrosis factor (ligand) superfamily, member 13b |
| Alternative Names |
ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit |
| Abbreviation |
TNFSF13B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
93 |
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| Range % |
90-95 |
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| 1:8 |
Average % |
92 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-99 |
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| EDTA plasma (n=4) |
90 |
87-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.912 |
2.953 |
2.933 |
2.819 |
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| 10 |
2.142 |
2.175 |
2.159 |
2.045 |
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| 5 |
1.295 |
1.312 |
1.304 |
1.190 |
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| 2.5 |
0.727 |
0.695 |
0.711 |
0.597 |
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| 1.25 |
0.426 |
0.443 |
0.435 |
0.321 |
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| 0.625 |
0.297 |
0.312 |
0.305 |
0.191 |
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| 0.312 |
0.247 |
0.261 |
0.254 |
0.140 |
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| 0 |
0.112 |
0.115 |
0.114 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
