Human Baculoviral IAP repeat-containing protein 3(BIRC3) ELISA kit
Product Details
| Target Name |
baculoviral IAP repeat-containing 3 |
| Alternative Names |
AIP 1 ELISA Kit; AIP1 ELISA Kit; API 2 ELISA Kit; API2 ELISA Kit; Apoptosis inhibitor 2 ELISA Kit; Baculoviral IAP repeat containing 3 ELISA Kit; Baculoviral IAP repeat containing protein 3 ELISA Kit; Baculoviral IAP repeat-containing protein 3 ELISA Kit; BIRC 3 ELISA Kit; BIRC3 ELISA Kit; BIRC3_HUMAN ELISA Kit; C IAP2 ELISA Kit; C-IAP2 ELISA Kit; Cellular inhibitor of apoptosis 2 ELISA Kit; cellular inhibitor of apoptosis protein 2 ELISA Kit; CIAP 2 ELISA Kit; CIAP2 ELISA Kit; HAIP 1 ELISA Kit; HAIP1 ELISA Kit; HIAP 1 ELISA Kit; HIAP-1 ELISA Kit; HIAP1 ELISA Kit; IAP homolog C ELISA Kit; IAP-1 ELISA Kit; Inhibitor of apoptosis protein 1 ELISA Kit; MALT 2 ELISA Kit; MALT2 ELISA Kit; Mammalian IAP homolog C ELISA Kit; MIHC ELISA Kit; RING finger protein 49 ELISA Kit; RNF49 ELISA Kit; TNFR2 TRAF signaling complex protein 1 ELISA Kit; TNFR2 TRAF signalling complex protein ELISA Kit; TNFR2-TRAF-signaling complex protein 1 ELISA Kit |
| Abbreviation |
BIRC3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
37.5 pg/mL-2400 pg/mL |
| Sensitivity |
9.4 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
91 |
|
|
| Range % |
86-94 |
|
|
| 1:2 |
Average % |
92 |
|
|
| Range % |
87-95 |
|
|
| 1:4 |
Average % |
94 |
|
|
| Range % |
87-98 |
|
|
| 1:8 |
Average % |
103 |
|
|
| Range % |
96-107 |
|
|
|
| Recovery |
| The recovery of human BIRC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
91 |
86-94 |
|
|
| EDTA plasma (n=4) |
103 |
98-107 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 2400 |
2.154 |
2.274 |
2.214 |
2.112 |
|
|
| 1200 |
1.268 |
1.318 |
1.293 |
1.191 |
|
|
| 600 |
0.741 |
0.798 |
0.770 |
0.668 |
|
|
| 300 |
0.443 |
0.460 |
0.452 |
0.350 |
|
|
| 150 |
0.292 |
0.285 |
0.289 |
0.187 |
|
|
| 75 |
0.167 |
0.174 |
0.171 |
0.069 |
|
|
| 37.5 |
0.132 |
0.139 |
0.136 |
0.034 |
|
|
| 0 |
0.101 |
0.102 |
0.102 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
