Human Beta-adrenergic receptor kinase 1(ADRBK1) ELISA kit
Product Details
| Target Name |
adrenergic, beta, receptor kinase 1 |
| Alternative Names |
ADRBK1 ELISA Kit; Adrenergic beta receptor kinase 1 ELISA Kit; ARBK1_HUMAN ELISA Kit; BARK ELISA Kit; BARK1 ELISA Kit; Beta adrenergic receptor kinase 1 ELISA Kit; Beta ARK 1 ELISA Kit; Beta ARK1 ELISA Kit; Beta-adrenergic receptor kinase 1 ELISA Kit; Beta-ARK-1 ELISA Kit; FLJ16718 ELISA Kit; G protein coupled receptor kinase 2 ELISA Kit; G-protein coupled receptor kinase 2 ELISA Kit; GRK2 ELISA Kit |
| Abbreviation |
ADRBK1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
18.75 pg/mL-1200 pg/mL |
| Sensitivity |
4.68 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADRBK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
97 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
94 |
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| Range % |
91-99 |
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| Recovery |
| The recovery of human ADRBK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
87-93 |
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| EDTA plasma (n=4) |
99 |
93-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1200 |
2.036 |
2.166 |
2.101 |
1.927 |
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| 600 |
1.373 |
1.405 |
1.389 |
1.215 |
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| 300 |
0.997 |
0.957 |
0.977 |
0.803 |
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| 150 |
0.633 |
0.675 |
0.654 |
0.480 |
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| 75 |
0.392 |
0.402 |
0.397 |
0.223 |
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| 37.5 |
0.294 |
0.284 |
0.289 |
0.115 |
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| 18.75 |
0.241 |
0.233 |
0.237 |
0.063 |
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| 0 |
0.167 |
0.180 |
0.174 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
