| Target Name |
blca-4 |
| Abbreviation |
BLCA-4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
87 |
|
| Range % |
84-91 |
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| 1:2 |
Average % |
99 |
|
| Range % |
96-103 |
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| 1:4 |
Average % |
104 |
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| Range % |
101-107 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
87 |
84-90 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.595 |
2.540 |
2.568 |
2.452 |
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| 20 |
2.208 |
2.365 |
2.287 |
2.171 |
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| 10 |
1.823 |
1.804 |
1.814 |
1.698 |
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| 5 |
1.195 |
1.175 |
1.185 |
1.069 |
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| 2.5 |
0.698 |
0.678 |
0.688 |
0.572 |
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| 1.25 |
0.418 |
0.432 |
0.425 |
0.309 |
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| 0.625 |
0.271 |
0.276 |
0.274 |
0.158 |
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| 0 |
0.115 |
0.117 |
0.116 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
