Human bone alkaline phosphatase,BALP ELISA Kit
Product Details
| Description |
The Human bone alkaline phosphatase (BALP) ELISA Kit quantitates BALP levels in human serum, plasma, or tissue homogenates. BALP is an enzyme that promotes bone mineralization by inactivating pyrophosphate and osteopontin, which both are inhibitors of bone mineralization. It is essential in determining the rate of bone formation and turnover. BALP is regarded as a marker of bone formation. Recent studies demonstrated BALP plays a crucial role in the pathogenesis of vascular calcification and identified BALP as a promising predictor of mortality in chronic kidney disease (CKD). This kit employs the sandwich-ELISA mechanism in conjugation with BALP antibody-BALP antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of BALP in the samples. The kit is catheterized with high sensitivity, strong specificity, good linearity, recovery of 89%-108%, precision less than 10%, and lot-to-lot consistency. Refer to the product instructions for more validation i... Read more |
| Target Name |
bone alkaline phosphatase,BALP |
| Alternative Names |
AKP2 ELISA Kit; Alkaline phosphatase liver/bone/kidney ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit; Alkaline phosphatase tissue nonspecific isozyme ELISA Kit; Alkaline phosphatase, tissue-nonspecific isozyme ELISA Kit; Alkaline phosphomonoesterase ELISA Kit; Alpl ELISA Kit; AP TNAP ELISA Kit; AP-TNAP ELISA Kit; APTNAP ELISA Kit; BAP ELISA Kit; FLJ40094 ELISA Kit; FLJ93059 ELISA Kit; Glycerophosphatase ELISA Kit; HOPS ELISA Kit; Liver/bone/kidney type alkaline phosphatase ELISA Kit; MGC161443 ELISA Kit; MGC167935 ELISA Kit; PHOA ELISA Kit; PPBT_HUMAN ELISA Kit; Tissue non specific alkaline phosphatase ELISA Kit; Tissue nonspecific ALP ELISA Kit; TNAP ELISA Kit; TNSALP ELISA Kit |
| Abbreviation |
BALP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
100 |
|
| Range % |
96-107 |
|
| 1:2 |
Average % |
89 |
|
| Range % |
85-96 |
|
| 1:4 |
Average % |
92 |
|
| Range % |
86-98 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
88-98 |
|
|
| Recovery |
| The recovery of human BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-97 |
|
| EDTA plasma (n=4) |
102 |
97-108 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 200 |
2.492 |
2.392 |
2.442 |
2.311 |
|
| 100 |
1.912 |
1.812 |
1.862 |
1.731 |
|
| 50 |
1.283 |
1.183 |
1.233 |
1.102 |
|
| 25 |
0.699 |
0.679 |
0.689 |
0.558 |
|
| 12.5 |
0.426 |
0.416 |
0.421 |
0.290 |
|
| 6.25 |
0.334 |
0.324 |
0.329 |
0.198 |
|
| 3.12 |
0.218 |
0.208 |
0.213 |
0.082 |
|
| 0 |
0.132 |
0.129 |
0.131 |
|
|
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BALP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled BALP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
