Human Bone Morphogenetic Protein 6,BMP-6 ELISA Kit
Product Details
| Target Name |
bone morphogenetic protein 6 |
| Alternative Names |
BMP-6 ELISA Kit; Bmp6 ELISA Kit; BMP6_HUMAN ELISA Kit; Bone morphogenetic protein 6 ELISA Kit; Bone Morphogenic Protein 6 ELISA Kit; Decapentaplegic vegetal related ELISA Kit; DVR6 ELISA Kit; HGNC:12686 ELISA Kit; TGFB related vegetal related growth factor ELISA Kit; Transforming growth factor beta ELISA Kit; Vegetal related (TGFB related) cytokine ELISA Kit; Vegetal related growth factor (TGFB related) ELISA Kit; Vg related sequence ELISA Kit; VG-1-R ELISA Kit; VG-1-related protein ELISA Kit; Vg1 related sequence ELISA Kit; VGR ELISA Kit; VGR-1 ELISA Kit; VGR1 ELISA Kit |
| Abbreviation |
BMP6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-102 |
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| 1:4 |
Average % |
91 |
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| Range % |
84-105 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-102 |
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| Recovery |
| The recovery of human BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.280 |
2.217 |
2.249 |
2.154 |
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| 1000 |
1.925 |
1.987 |
1.956 |
1.861 |
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| 500 |
1.515 |
1.624 |
1.570 |
1.475 |
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| 250 |
1.048 |
1.008 |
1.028 |
0.933 |
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| 125 |
0.596 |
0.583 |
0.590 |
0.495 |
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| 62.5 |
0.374 |
0.355 |
0.365 |
0.270 |
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| 31.25 |
0.241 |
0.252 |
0.247 |
0.152 |
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| 0 |
0.096 |
0.093 |
0.095 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
