Human Bone morphogenetic protein 7,BMP-7 ELISA Kit
Product Details
| Target Name |
bone morphogenetic protein 7 |
| Alternative Names |
BMP 7 ELISA Kit; BMP-7 ELISA Kit; Bmp7 ELISA Kit; BMP7_HUMAN ELISA Kit; Bone morphogenetic protein 7 ELISA Kit; Bone morphogenic protein 7 ELISA Kit; Eptotermin alfa ELISA Kit; OP 1 ELISA Kit; OP-1 ELISA Kit; OP1 ELISA Kit; Osteogenic protein 1 ELISA Kit |
| Abbreviation |
BMP7 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:2 |
Average % |
102 |
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| Range % |
98-107 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
94 |
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| Range % |
87-98 |
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| Recovery |
| The recovery of human BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-100 |
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| EDTA plasma (n=4) |
88 |
82-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.548 |
2.687 |
2.618 |
2.449 |
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| 500 |
1.982 |
1.824 |
1.903 |
1.734 |
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| 250 |
1.138 |
1.167 |
1.153 |
0.984 |
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| 125 |
0.653 |
0.668 |
0.661 |
0.492 |
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| 62.5 |
0.405 |
0.412 |
0.409 |
0.240 |
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| 31.2 |
0.287 |
0.274 |
0.281 |
0.112 |
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| 15.6 |
0.220 |
0.226 |
0.223 |
0.054 |
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| 0 |
0.168 |
0.169 |
0.169 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
