Human brain natriuretic peptide,BNP ELISA Kit
Product Details
| Description |
The product CSB-E07970h is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of human brain natriuretic peptide (BNP) in biological samples, including serum, urine, tissue homogenates, and cell culture supernates. BNP is a hormone secreted by cardiomyocytes in the heart ventricles. It is released in response to ventricular blood volume expansion and the increased well stress of cardiac myocytes. Based on the strong association with prognosis in patients with heart failure (HF), BNP levels have been widely used as a marker of congestion in the diagnosis of congestive heart failure (CHF). This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human BNP in the sample. Standards and samples are respectively added to the microplate wells pre-coated with anti-human BNP antibody. Biotin-labeled BNP antibody, HRP-avidin, TMB substrate are pipped into the microplate in turn. BNP in the sample or standard is captured by the imm... Read more |
| Target Name |
brain natriuretic peptide,BNP |
| Alternative Names |
ANFB_HUMAN ELISA Kit; BNP ELISA Kit; BNP(1-32) ELISA Kit; BNP(5-29) ELISA Kit; BNP-32 ELISA Kit; Brain type natriuretic peptide ELISA Kit; Gamma brain natriuretic peptide ELISA Kit; Gamma-brain natriuretic peptide ELISA Kit; Natriuretic peptide precursor B ELISA Kit; nppb ELISA Kit; NT proBNP ELISA Kit |
| Abbreviation |
BNP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, urine, tissue homogenates, cell culture supernates |
| Detection Range |
93.75 pg/mL-6000 pg/mL |
| Sensitivity |
23.43 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
81-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
91-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 6000 |
2.311 |
2.256 |
2.284 |
2.179 |
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| 3000 |
1.905 |
1.876 |
1.891 |
1.786 |
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| 1500 |
1.548 |
1.447 |
1.498 |
1.393 |
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| 750 |
1.102 |
1.059 |
1.081 |
0.976 |
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| 375 |
0.871 |
0.857 |
0.864 |
0.759 |
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| 187.5 |
0.502 |
0.490 |
0.496 |
0.391 |
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| 93.75 |
0.313 |
0.297 |
0.305 |
0.200 |
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| 0 |
0.109 |
0.101 |
0.105 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BNP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled BNP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
