Human C-C motif chemokine 27 (CCL27) ELISA Kit
Product Details
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| Target Name |
C-C motif chemokine 27 (CCL27) |
| Alternative Names |
ALP ELISA Kit; C-C motif chemokine 27 ELISA Kit; CC chemokine ILC ELISA Kit; Ccl27 ELISA Kit; CCL27_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 27 ELISA Kit; CTACK ELISA Kit; CTAK ELISA Kit; Cutaneous T cell attracting chemokine ELISA Kit; Cutaneous T-cell-attracting chemokine ELISA Kit; ESkine ELISA Kit; IL 11 R alpha locus chemokine ELISA Kit; IL-11 R-alpha-locus chemokine ELISA Kit; ILC ELISA Kit; PESKY ELISA Kit; SCYA27 ELISA Kit; Skinkine ELISA Kit; Small inducible cytokine A27 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 27 ELISA Kit; Small-inducible cytokine A27 ELISA Kit |
| Abbreviation |
CCL27 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.313 ng/mL-20 ng/mL |
| Sensitivity |
0.043 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCL27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-88 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
104 |
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| Range % |
101-107 |
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| 1:8 |
Average % |
89 |
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| Range % |
85-93 |
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| Recovery |
| The recovery of human CCL27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
94-100 |
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| EDTA plasma (n=4) |
105 |
101-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.715 |
2.768 |
2.742 |
2.589 |
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| 10 |
2.097 |
2.014 |
2.056 |
1.903 |
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| 5 |
1.276 |
1.234 |
1.255 |
1.102 |
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| 2.5 |
0.752 |
0.768 |
0.760 |
0.607 |
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| 1.25 |
0.481 |
0.498 |
0.490 |
0.337 |
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| 0.625 |
0.327 |
0.310 |
0.319 |
0.166 |
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| 0.313 |
0.257 |
0.244 |
0.251 |
0.098 |
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| 0 |
0.156 |
0.150 |
0.153 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
