Human C-type lectin domain family 9 member A(CLEC9A) ELISA kit
Product Details
| Target Name |
C-type lectin domain family 9, member A |
| Alternative Names |
C type lectin domain family 9; member A ELISA Kit; C-type lectin domain family 9 member A ELISA Kit; CLC9A_HUMAN ELISA Kit; CLEC9A ELISA Kit; DNGR1 ELISA Kit; HEEE9341 ELISA Kit; PRO34046 ELISA Kit; UNQ9341 ELISA Kit |
| Abbreviation |
CLEC9A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC9A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
85-93 |
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| 1:2 |
Average % |
104 |
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| Range % |
100-108 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-93 |
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| 1:8 |
Average % |
108 |
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| Range % |
104-112 |
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| Recovery |
| The recovery of human CLEC9A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
84-93 |
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| EDTA plasma (n=4) |
106 |
103-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.273 |
2.109 |
2.191 |
2.038 |
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| 10 |
1.575 |
1.604 |
1.590 |
1.437 |
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| 5 |
1.179 |
1.205 |
1.192 |
1.039 |
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| 2.5 |
0.825 |
0.825 |
0.825 |
0.672 |
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| 1.25 |
0.525 |
0.532 |
0.529 |
0.376 |
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| 0.625 |
0.375 |
0.368 |
0.372 |
0.219 |
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| 0.312 |
0.264 |
0.261 |
0.263 |
0.110 |
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| 0 |
0.154 |
0.152 |
0.153 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
