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Human C-type mannose receptor 2(MRC2) ELISA kit

 

Product Details

Target Name mannose receptor, C type 2
Alternative Names C-type lectin domain family 13 member E ELISA Kit; C-type mannose receptor 2 ELISA Kit; CD 280 ELISA Kit; CD280 ELISA Kit; CD280 antigen ELISA Kit; CLEC 13E ELISA Kit; CLEC13E ELISA Kit; ENDO 180 ELISA Kit; ENDO180 ELISA Kit; Endocytic receptor (macrophage mannose receptor family) ELISA Kit; Endocytic receptor 180 ELISA Kit; FLJ35911 ELISA Kit; KIAA0709 ELISA Kit; Macrophage mannose receptor 2 ELISA Kit; Mannose receptor C type 2 ELISA Kit; MRC 2 ELISA Kit; MRC2 ELISA Kit; MRC2_HUMAN ELISA Kit; UPAR-associated protein ELISA Kit; UPARAP ELISA Kit; Urokinase plasminogen activator receptor associated protein ELISA Kit; Urokinase receptor associated protein ELISA Kit; Urokinase receptor-associated protein ELISA Kit; Urokinase-type plasminogen activator receptor-associated protein ELISA Kit
Abbreviation MRC2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MRC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-90  
1:2 Average % 94  
Range % 88-105  
1:4 Average % 99  
Range % 90-110  
1:8 Average % 94  
Range % 80-100  
Recovery
The recovery of human MRC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 80-98  
EDTA plasma (n=4) 99 92-110  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.045 1.952 1.999 1.810  
5 1.612 1.706 1.659 1.470  
2.5 1.312 1.240 1.276 1.087  
1.25 0.841 0.839 0.840 0.651  
0.625 0.650 0.605 0.628 0.439  
0.312 0.446 0.415 0.431 0.242  
0.156 0.275 0.269 0.272 0.083  
0 0.191 0.187 0.189    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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