Human C4b-binding protein beta chain(C4BPB) ELISA kit
Product Details
| Target Name |
complement component 4 binding protein, beta |
| Alternative Names |
C4b binding protein beta chain ELISA Kit; C4b-binding protein beta chain ELISA Kit; C4BP ELISA Kit; C4BPB ELISA Kit; C4BPB_HUMAN ELISA Kit; Complement component 4 binding protein beta ELISA Kit; Complement component 4 binding protein beta chain ELISA Kit; Complement component 4-binding protein beta ELISA Kit; OTTHUMP00000034288 ELISA Kit; OTTHUMP00000034289 ELISA Kit; OTTHUMP00000034375 ELISA Kit; OTTHUMP0000003474 ELISA Kit; Proline rich protein ELISA Kit; PRP ELISA Kit |
| Abbreviation |
C4BPB |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
96 |
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| Range % |
91-108 |
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| 1:2000 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:4000 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8000 |
Average % |
97 |
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| Range % |
91-112 |
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| Recovery |
| The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-105 |
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| EDTA plasma (n=4) |
95 |
89-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.905 |
2.875 |
2.890 |
2.755 |
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| 100 |
2.489 |
2.473 |
2.481 |
2.346 |
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| 50 |
1.734 |
1.750 |
1.742 |
1.607 |
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| 25 |
0.948 |
0.957 |
0.953 |
0.818 |
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| 12.5 |
0.512 |
0.506 |
0.509 |
0.374 |
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| 6.25 |
0.464 |
0.471 |
0.468 |
0.333 |
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| 3.125 |
0.230 |
0.234 |
0.232 |
0.097 |
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| 0 |
0.133 |
0.136 |
0.135 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
