Human CAMPATH-1 antigen(CD52) ELISA kit
Product Details
| Target Name |
CD52 molecule |
| Alternative Names |
Cambridge pathology 1 antigen ELISA Kit; CAMPATH 1 ELISA Kit; CAMPATH 1 antigen ELISA Kit; CAMPATH-1 antigen ELISA Kit; CD 52 ELISA Kit; CD52 ELISA Kit; CD52 antigen ELISA Kit; CD52 molecule ELISA Kit; CD52_HUMAN ELISA Kit; CDw52 ELISA Kit; CDW52 antigen ELISA Kit; Epididymal secretory protein E5 ELISA Kit; Epididymis secretory sperm binding protein Li 171mP ELISA Kit; HE 5 ELISA Kit; He5 ELISA Kit; Human epididymis-specific protein 5 ELISA Kit |
| Abbreviation |
CD52 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
78 ng/mL-5000 ng/mL |
| Sensitivity |
19.5 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD52 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-102 |
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| Recovery |
| The recovery of human CD52 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
80-100 |
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| EDTA plasma (n=4) |
98 |
91-110 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
1.975 |
1.952 |
1.964 |
1.870 |
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| 2500 |
1.612 |
1.586 |
1.599 |
1.505 |
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| 1250 |
1.272 |
1.171 |
1.222 |
1.128 |
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| 625 |
0.984 |
0.932 |
0.958 |
0.864 |
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| 312 |
0.665 |
0.629 |
0.647 |
0.553 |
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| 156 |
0.398 |
0.368 |
0.383 |
0.289 |
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| 78 |
0.193 |
0.201 |
0.197 |
0.103 |
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| 0 |
0.097 |
0.091 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
