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Human Carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1) ELISA kit

Product Details

Target Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Alternative Names Antigen CD66 ELISA Kit; BGP 1 ELISA Kit; BGP ELISA Kit; BGP-1 ELISA Kit; BGPI ELISA Kit; Biliary glycoprotein 1 ELISA Kit; Biliary glycoprotein adhesion molecule ELISA Kit; Biliary glycoprotein ELISA Kit; Carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit; CD66a ELISA Kit; CD66a antigen ELISA Kit; CEACAM1 ELISA Kit; CEAM1_HUMAN ELISA Kit; meconium antigen 100 ELISA Kit
Abbreviation CEACAM1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8>      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10>      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEACAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 85-95  
1:2 Average % 93  
Range % 90-100  
1:4 Average % 95  
Range % 89-99  
1:8 Average % 94  
Range % 82-98  
Recovery
The recovery of human CEACAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-103  
EDTA plasma (n=4) 95 87-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.580 2.542 2.561 2.362  
25 1.774 1.800 1.787 1.588  
12.5 1.055 1.104 1.080 0.881  
6.25 0.628 0.635 0.632 0.433  
3.12 0.514 0.507 0.511 0.312  
1.56 0.368 0.361 0.365 0.166  
0.78 0.273 0.284 0.279 0.080  
0 0.201 0.197 0.199    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days