Human Carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1) ELISA kit
Product Details
Target Name |
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) |
Alternative Names |
Antigen CD66 ELISA Kit; BGP 1 ELISA Kit; BGP ELISA Kit; BGP-1 ELISA Kit; BGPI ELISA Kit; Biliary glycoprotein 1 ELISA Kit; Biliary glycoprotein adhesion molecule ELISA Kit; Biliary glycoprotein ELISA Kit; Carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit; CD66a ELISA Kit; CD66a antigen ELISA Kit; CEACAM1 ELISA Kit; CEAM1_HUMAN ELISA Kit; meconium antigen 100 ELISA Kit |
Abbreviation |
CEACAM1 |
Species |
Homo sapiens (Human) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
0.78 ng/mL-50 ng/mL |
Sensitivity |
0.195 ng/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology |
Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8>
| |
|
|
Three samples of known concentration were tested twenty times on one plate to assess. |
|
Inter-assay Precision (Precision between assays): CV%<10>
| |
|
|
Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
|
|
|
|
|
|
|
Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of human CEACAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:1 |
Average % |
87 |
|
Range % |
85-95 |
|
1:2 |
Average % |
93 |
|
Range % |
90-100 |
|
1:4 |
Average % |
95 |
|
Range % |
89-99 |
|
1:8 |
Average % |
94 |
|
Range % |
82-98 |
|
|
Recovery |
The recovery of human CEACAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
95 |
89-103 |
|
EDTA plasma (n=4) |
95 |
87-100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
50 |
2.580 |
2.542 |
2.561 |
2.362 |
|
25 |
1.774 |
1.800 |
1.787 |
1.588 |
|
12.5 |
1.055 |
1.104 |
1.080 |
0.881 |
|
6.25 |
0.628 |
0.635 |
0.632 |
0.433 |
|
3.12 |
0.514 |
0.507 |
0.511 |
0.312 |
|
1.56 |
0.368 |
0.361 |
0.365 |
0.166 |
|
0.78 |
0.273 |
0.284 |
0.279 |
0.080 |
|
0 |
0.201 |
0.197 |
0.199 |
|
|
|
|
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
7-14 working days |