| Description |
The Human Caspase 1 (Casp-1) ELISA Kit is suitable for quantitative detection of Casp-1 in serum, plasma, and tissue homogenates. Samples or standards are added into the microwells and bind to the anti-human Casp-1 antibody immobilized on the microplate. A sandwich is formed by the addition of the biotin-conjugated Casp-1 antibody. The TMB substrate solution is added that reacts with the enzyme-antibody-target complex to elicit a chromogenic reaction. The intensity of the solution color is directly proportional to the concentration of Casp-1 present in the original specimen. This kit has been verified with a wide detection range (12.5 pg/mL-800 pg/mL), high sensitivity, excellent specificity, precision less than 10%, good linearity, high recovery, and good consistency between lots. Refer to the product instructions for more verification details. Casp-1 is synthesized as an inactive zymogen that is recruited and activated by inflammasomes. It plays a crucial role in cell immunity
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| Target Name |
caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| Alternative Names |
CASP-1 ELISA Kit; CASP1 ELISA Kit; CASP1_HUMAN ELISA Kit; Caspase 1 ELISA Kit; Caspase-1 subunit p10 ELISA Kit; ICE ELISA Kit; IL-1 beta-converting enzyme ELISA Kit; IL-1BC ELISA Kit; IL1 beta converting enzyme ELISA Kit; IL1B convertase ELISA Kit; Interleukin 1 beta convertase ELISA Kit; Interleukin 1B converting enzyme ELISA Kit; Interleukin-1 beta convertase ELISA Kit; Interleukin-1 beta-converting enzyme ELISA Kit; p45 ELISA Kit |
| Abbreviation |
CASP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.1 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
81-92 |
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| 1:2 |
Average % |
101 |
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| Range % |
96-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
103 |
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| Range % |
97-108 |
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| Recovery |
| The recovery of human Casp-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
101 |
97-105 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.112 |
1.984 |
2.048 |
1.920 |
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| 400 |
1.634 |
1.537 |
1.586 |
1.458 |
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| 200 |
1.124 |
1.085 |
1.105 |
0.977 |
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| 100 |
0.718 |
0.693 |
0.706 |
0.578 |
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| 50 |
0.389 |
0.394 |
0.392 |
0.264 |
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| 25 |
0.262 |
0.274 |
0.268 |
0.140 |
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| 12.5 |
0.189 |
0.182 |
0.186 |
0.058 |
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| 0 |
0.128 |
0.127 |
0.128 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human Casp-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled Casp-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
