Human Caspase-7(CASP7) ELISA kit
Product Details
| Target Name |
caspase 7, apoptosis-related cysteine peptidase |
| Alternative Names |
apoptosis-related cysteine peptidase ELISA Kit; Apoptotic protease Mch-3 ELISA Kit; CASP-7 ELISA Kit; CASP7 ELISA Kit; CASP7_HUMAN ELISA Kit; Caspase-7 subunit p11 ELISA Kit; CMH-1 ELISA Kit; ICE-LAP3 ELISA Kit; ICE-like apoptotic protease 3 ELISA Kit |
| Abbreviation |
CASP7 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell lysates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CASP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
85-98 |
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| 1:2 |
Average % |
103 |
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| Range % |
95-107 |
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| 1:4 |
Average % |
90 |
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| Range % |
83-94 |
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| 1:8 |
Average % |
98 |
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| Range % |
90-102 |
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| Recovery |
| The recovery of human CASP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-96 |
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| EDTA plasma (n=4) |
99 |
91-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.203 |
2.156 |
2.180 |
2.023 |
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| 2000 |
1.377 |
1.433 |
1.405 |
1.248 |
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| 1000 |
0.902 |
0.915 |
0.909 |
0.752 |
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| 500 |
0.504 |
0.510 |
0.507 |
0.350 |
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| 250 |
0.353 |
0.347 |
0.350 |
0.193 |
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| 125 |
0.272 |
0.266 |
0.269 |
0.112 |
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| 62.5 |
0.234 |
0.225 |
0.230 |
0.073 |
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| 0 |
0.158 |
0.156 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
