Human Cathepsin K,cath-K ELISA Kit
Product Details
| Target Name |
cathepsin K |
| Alternative Names |
Cathepsin K ELISA Kit; Cathepsin O ELISA Kit; Cathepsin O1 ELISA Kit; Cathepsin O2 ELISA Kit; Cathepsin X ELISA Kit; CATK_HUMAN ELISA Kit; CTS02 ELISA Kit; Ctsk ELISA Kit; CTSO ELISA Kit; CTSO1 ELISA Kit; CTSO2 ELISA Kit; MGC23107 ELISA Kit; PKND ELISA Kit; PYCD ELISA Kit |
| Abbreviation |
CTSK |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
97 |
|
| Range % |
92-105 |
|
| 1:2 |
Average % |
92 |
|
| Range % |
88-98 |
|
| 1:4 |
Average % |
85 |
|
| Range % |
80-90 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
91-100 |
|
|
| Recovery |
| The recovery of human cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-102 |
|
| EDTA plasma (n=4) |
95 |
88-99 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
1.886 |
1.822 |
1.854 |
1.735 |
|
| 250 |
1.568 |
1.489 |
1.529 |
1.410 |
|
| 125 |
1.213 |
1.201 |
1.207 |
1.088 |
|
| 62.5 |
0.976 |
0.939 |
0.958 |
0.839 |
|
| 31.2 |
0.652 |
0.624 |
0.638 |
0.519 |
|
| 15.6 |
0.348 |
0.325 |
0.337 |
0.218 |
|
| 7.8 |
0.206 |
0.199 |
0.203 |
0.084 |
|
| 0 |
0.121 |
0.117 |
0.119 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
