Human CD81 antigen(CD81) ELISA kit
Product Details
| Description |
The human CD81 antigen (CD81) ELISA kit allows for the quantitative determination of CD81 levels in human serum, plasma, tissue homogenates, or cell lysates. It can detect human CD81 in the range of 0.156 ng/mL-10 ng/mL. Its minimum detection dose of human CD81 is typically less than 0.039 ng/ml. This assay has high sensitivity and excellent specificity for the detection of human CD81. No significant cross-reactivity or interference between human CD81 and analogs was observed. This kit has also been validated in other parameters, including precision, linearity, and recovery. This kit contains all reagents necessary for quantifying human CD81 concentrations. In this assay, an anti-human CD81 antibody has been pre-coated on the microplate. Standards and samples are pipetted into the wells. After thorough washing, a biotin-conjugated CD81 antibody is added to the wells. CD81 in the samples or in the standards is sandwiched between these two antibodies. Following washing, HRP-avidin and TM... Read more |
| Target Name |
CD81 molecule |
| Alternative Names |
26 kDa cell surface protein TAPA 1 ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; 26 kDa cell surface protein TAPA1 ELISA Kit; CD 81 ELISA Kit; CD81 ELISA Kit; CD81 antigen (target of antiproliferative ELISA Kit 1) ELISA Kit; CD81 antigen ELISA Kit; CD81 molecule ELISA Kit; CD81_HUMAN ELISA Kit; CVID6 ELISA Kit; S5.7 ELISA Kit; TAPA 1 ELISA Kit; TAPA1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; Tetraspanin 28 ELISA Kit; Tetraspanin-28 ELISA Kit; Tetraspanin28 ELISA Kit; Tspan 28 ELISA Kit; Tspan-28 ELISA Kit; Tspan28 ELISA Kit |
| Abbreviation |
CD81 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
92 |
|
| Range % |
88-101 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
92-105 |
|
| 1:4 |
Average % |
92 |
|
| Range % |
87-100 |
|
| 1:5 |
Average % |
95 |
|
| Range % |
90-100 |
|
|
| Recovery |
| The recovery of human CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
95-100 |
|
| EDTA plasma (n=4) |
98 |
94-102 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.185 |
2.064 |
2.125 |
1.977 |
|
| 5 |
1.407 |
1.381 |
1.394 |
1.246 |
|
| 2.5 |
0.807 |
0.790 |
0.799 |
0.651 |
|
| 1.25 |
0.451 |
0.434 |
0.443 |
0.295 |
|
| 0.625 |
0.326 |
0.352 |
0.339 |
0.191 |
|
| 0.312 |
0.269 |
0.273 |
0.271 |
0.123 |
|
| 0.156 |
0.197 |
0.204 |
0.201 |
0.053 |
|
| 0 |
0.149 |
0.146 |
0.148 |
|
|
|
|
| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human CD81antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CD81 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
