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Human Cementum Protein 1 (CEMP-1) ELISA Kit

Product Details

Target Name cementum protein 1
Alternative Names Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
Abbreviation CEMP1
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-95  
1:2 Average % 101  
Range % 97-105  
1:4 Average % 83  
Range % 80-86  
1:8 Average % 107  
Range % 103-110  
Recovery
The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 91 84-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.658 2.697 2.678 2.528  
5 2.312 2.331 2.322 2.172  
2.5 1.871 1.892 1.882 1.732  
1.25 1.312 1.365 1.339 1.189  
0.625 0.788 0.797 0.793 0.643  
0.312 0.442 0.472 0.457 0.307  
0.156 0.281 0.272 0.277 0.127  
0 0.151 0.149 0.150    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days