Human Cementum Protein 1 (CEMP-1) ELISA Kit
Product Details
| Target Name |
cementum protein 1 |
| Alternative Names |
Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit |
| Abbreviation |
CEMP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:8 |
Average % |
107 |
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| Range % |
103-110 |
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| Recovery |
| The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
91 |
84-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.658 |
2.697 |
2.678 |
2.528 |
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| 5 |
2.312 |
2.331 |
2.322 |
2.172 |
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| 2.5 |
1.871 |
1.892 |
1.882 |
1.732 |
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| 1.25 |
1.312 |
1.365 |
1.339 |
1.189 |
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| 0.625 |
0.788 |
0.797 |
0.793 |
0.643 |
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| 0.312 |
0.442 |
0.472 |
0.457 |
0.307 |
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| 0.156 |
0.281 |
0.272 |
0.277 |
0.127 |
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| 0 |
0.151 |
0.149 |
0.150 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
