| Target Name |
retinoic acid receptor responder (tazarotene induced) 2 |
| Alternative Names |
HP10433 ELISA Kit; RAR responsive protein TIG2 ELISA Kit; RAR-responsive protein TIG2 ELISA Kit; RARR2_HUMAN ELISA Kit; RARRES2 ELISA Kit; Retinoic acid receptor responder (tazarotene induced) 2 ELISA Kit; Retinoic acid receptor responder 2 ELISA Kit; Retinoic acid receptor responder protein 2 ELISA Kit; Tazarotene induced gene 2 protein ELISA Kit; Tazarotene-induced gene 2 protein ELISA Kit; TIG2 ELISA Kit |
| Abbreviation |
RARRES2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.2 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human chemerin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
96 |
|
| Range % |
90-103 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
91-105 |
|
| 1:4 |
Average % |
95 |
|
| Range % |
92-100 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
85-99 |
|
|
| Recovery |
| The recovery of human chemerin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
88-99 |
|
| EDTA plasma (n=4) |
96 |
90-101 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2000 |
2.492 |
2.452 |
2.472 |
2.295 |
|
| 1000 |
1.967 |
1.976 |
1.972 |
1.795 |
|
| 500 |
1.314 |
1.293 |
1.304 |
1.127 |
|
| 250 |
0.768 |
0.792 |
0.780 |
0.603 |
|
| 125 |
0.472 |
0.452 |
0.462 |
0.285 |
|
| 62.5 |
0.384 |
0.367 |
0.376 |
0.199 |
|
| 31.2 |
0.298 |
0.295 |
0.297 |
0.120 |
|
| 0 |
0.176 |
0.177 |
0.177 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
