Human Claudin-5(CLDN5) ELISA kit
Product Details
| Target Name |
claudin 5 |
| Alternative Names |
Androgen withdrawal and apoptosis induced protein RVP1 like ELISA Kit; AWAL ELISA Kit; BEC 1 ELISA Kit; BEC1 ELISA Kit; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) ELISA Kit; Claudin-5 ELISA Kit; Claudin5 ELISA Kit; CLD5_HUMAN ELISA Kit; CLDN 5 ELISA Kit; Cldn5 ELISA Kit; CPETR L1 ELISA Kit; CPETRL 1 ELISA Kit; CPETRL1 ELISA Kit; TMDVCF ELISA Kit; TMVCF ELISA Kit; Transmembrane protein deleted in VCFS ELISA Kit; Transmembrane protein deleted in velocardiofacial syndrome ELISA Kit |
| Abbreviation |
CLDN5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
100 |
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| Range % |
94-107 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-99 |
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| Recovery |
| The recovery of human CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
102 |
95-106 |
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| EDTA plasma (n=4) |
95 |
89-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.930 |
2.012 |
1.971 |
1.850 |
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| 1000 |
1.496 |
1.598 |
1.547 |
1.426 |
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| 500 |
1.053 |
1.068 |
1.061 |
0.940 |
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| 250 |
0.732 |
0.756 |
0.744 |
0.623 |
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| 125 |
0.444 |
0.435 |
0.440 |
0.319 |
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| 62.5 |
0.312 |
0.326 |
0.319 |
0.198 |
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| 31.25 |
0.217 |
0.201 |
0.209 |
0.088 |
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| 0 |
0.122 |
0.120 |
0.121 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
