Human cluster Of differentiation 82 (CD82) ELISA Kit
Product Details
| Target Name |
CD82 molecule |
| Alternative Names |
4F9 ELISA Kit; Antigen detected by monoclonal and ELISA Kit IA4 ELISA Kit; C33 ELISA Kit; C33 antigen ELISA Kit; CD 82 ELISA Kit; CD82 ELISA Kit; CD82 antigen ELISA Kit; CD82 molecule ELISA Kit; CD82_HUMAN ELISA Kit; GR 15 ELISA Kit; GR15 ELISA Kit; IA 4 ELISA Kit; IA4 ELISA Kit; Inducible membrane protein ELISA Kit; Inducible membrane protein R2 ELISA Kit; KAI 1 ELISA Kit; KAI1 ELISA Kit; Kangai 1 (suppression of tumorigenicity 6; prostate; CD82 antigen (R2 leukocyte antigen; antigen detected by monoclonal and ELISA Kit IA4)) ELISA Kit; Kangai 1 ELISA Kit; Kangai1 ELISA Kit; Leukocyte surface antigen R2 ELISA Kit; Metastasis suppressor Kangai 1 ELISA Kit; Metastasis suppressor Kangai-1 ELISA Kit; Metastasis suppressor Kangai1 ELISA Kit; Prostate cancer antimetastasis gene KAI1 ELISA Kit; R2 ELISA Kit; R2 leukocyte antigen ELISA Kit; SAR 2 ELISA Kit; SAR2 ELISA Kit; ST 6 ELISA Kit; ST6 ELISA Kit; Suppression of tumorigenicity 6 ELISA Kit; Suppression of tumorigenicity 6 prostate ELISA Kit; Suppressor of tumorigenicity 6 ELISA Kit; Suppressor of tumorigenicity 6 protein ELISA Kit; Tetraspanin 27 ELISA Kit; Tetraspanin-27 ELISA Kit; Tetraspanin27 ELISA Kit; Tspan 27 ELISA Kit; Tspan-27 ELISA Kit; Tspan27 ELISA Kit |
| Abbreviation |
CD82 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD82 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
88-98 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
92-101 |
|
| 1:4 |
Average % |
95 |
|
| Range % |
90-99 |
|
| 1:8 |
Average % |
84 |
|
| Range % |
80-88 |
|
|
| Recovery |
| The recovery of human CD82 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
104 |
99-110 |
|
| EDTA plasma (n=4) |
89 |
82-93 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.748 |
2.702 |
2.725 |
2.601 |
|
| 5 |
2.131 |
2.192 |
2.162 |
2.038 |
|
| 2.5 |
1.491 |
1.416 |
1.454 |
1.330 |
|
| 1.25 |
0.866 |
0.827 |
0.847 |
0.723 |
|
| 0.625 |
0.546 |
0.502 |
0.524 |
0.400 |
|
| 0.312 |
0.363 |
0.391 |
0.377 |
0.253 |
|
| 0.156 |
0.254 |
0.239 |
0.247 |
0.123 |
|
| 0 |
0.125 |
0.123 |
0.124 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
