| Target Name |
Coll2-1 |
| Abbreviation |
Coll2-1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
47 pg/mL-3000 pg/mL |
| Sensitivity |
11.72 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
85 |
|
| Range % |
80-90 |
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| 1:200 |
Average % |
98 |
|
| Range % |
90-105 |
|
| 1:400 |
Average % |
95 |
|
| Range % |
90-100 |
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| 1:800 |
Average % |
92 |
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| Range % |
87-97 |
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| Recovery |
| The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
83-95 |
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| EDTA plasma (n=4) |
98 |
92-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.513 |
2.487 |
2.500 |
2.417 |
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| 1500 |
1.847 |
1.794 |
1.821 |
1.738 |
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| 750 |
1.198 |
1.112 |
1.155 |
1.072 |
|
| 375 |
0.754 |
0.709 |
0.732 |
0.649 |
|
| 187.5 |
0.351 |
0.337 |
0.344 |
0.261 |
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| 94 |
0.198 |
0.183 |
0.191 |
0.108 |
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| 47 |
0.134 |
0.125 |
0.130 |
0.047 |
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| 0 |
0.084 |
0.081 |
0.083 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
