Human Collagen alpha-1(X) chain (COL10A1) ELISA kit
Product Details
| Target Name |
collagen, type X, alpha 1 |
| Alternative Names |
COAA1_HUMAN ELISA Kit; Col10a 1 ELISA Kit; COL10A1 ELISA Kit; Collagen alpha 1(X) chain ELISA Kit; Collagen alpha-1(X) chain ELISA Kit; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) ELISA Kit; Collagen type X alpha 1 ELISA Kit; Collagen X alpha 1 polypeptide ELISA Kit; CollagenX ELISA Kit; fa66d11 ELISA Kit; fb10c08 ELISA Kit; OTTHUMP00000040411 ELISA Kit; Procollagen type X alpha 1 ELISA Kit; Schmid metaphyseal chondrodysplasia ELISA Kit; wu:fa66d11 ELISA Kit; wu:fb10c08 ELISA Kit |
| Abbreviation |
COL10A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL10A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-106 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-95 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-103 |
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| Recovery |
| The recovery of human COL10A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-101 |
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| EDTA plasma (n=4) |
92 |
87-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
3.039 |
3.052 |
3.046 |
2.909 |
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| 10 |
2.647 |
2.658 |
2.653 |
2.516 |
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| 5 |
1.898 |
2.040 |
1.969 |
1.832 |
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| 2.5 |
1.211 |
1.262 |
1.237 |
1.100 |
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| 1.25 |
0.708 |
0.663 |
0.686 |
0.549 |
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| 0.625 |
0.425 |
0.406 |
0.416 |
0.279 |
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| 0.312 |
0.242 |
0.257 |
0.250 |
0.113 |
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| 0 |
0.138 |
0.136 |
0.137 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
