| Description |
The product CSB-E13446h is an ELISA kit specifically designed and validated for the quantitative detection of human Collagen, type III, alpha 1 (COL3A1) in multiple biological solutions, including serum, plasma, cell culture supernates, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for signal transduction research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which COL3A1 in the samples or standards are sandwiched between pre-coated COL3A1 antibody and Biotin-conjugated COL3A1 antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount... Read more |
| Target Name |
collagen, type III, alpha 1 |
| Alternative Names |
Alpha 1 type III collagen ELISA Kit; Alpha1 (III) collagen ELISA Kit; CO3A1_HUMAN ELISA Kit; COL 3A1 ELISA Kit; COL3A1 ELISA Kit; Collagen alpha 1(III) chain ELISA Kit; Collagen alpha-1(III) chain ELISA Kit; Collagen III alpha 1 chain precursor ELISA Kit; Collagen III alpha 1 polypeptide ELISA Kit; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) ELISA Kit; Collagen type III alpha 1 ELISA Kit; Collagen type III alpha 1 chain ELISA Kit; Collagen type III alpha ELISA Kit; Collagen; fetal ELISA Kit; EDS4A ELISA Kit; Ehlers Danlos syndrome type IV; autosomal dominant ELISA Kit; Fetal collagen ELISA Kit; Type III collagen ELISA Kit |
| Abbreviation |
COL3A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:4 |
Average % |
101 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-107 |
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| Recovery |
| The recovery of human COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
85-98 |
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| EDTA plasma (n=4) |
109 |
102-115 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.484 |
2.575 |
2.530 |
2.381 |
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| 5 |
1.788 |
1.889 |
1.839 |
1.690 |
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| 2.5 |
1.256 |
1.365 |
1.311 |
1.162 |
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| 1.25 |
0.732 |
0.766 |
0.749 |
0.600 |
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| 0.625 |
0.417 |
0.450 |
0.434 |
0.285 |
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| 0.312 |
0.274 |
0.284 |
0.279 |
0.130 |
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| 0.156 |
0.248 |
0.250 |
0.249 |
0.100 |
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| 0 |
0.146 |
0.151 |
0.149 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human COL3A1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled COL3A1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
