Human Complement C1s subcomponent(C1S) ELISA kit
Product Details
| Target Name |
complement component 1, s subcomponent |
| Alternative Names |
Basic proline rich peptide IB 1 ELISA Kit; C1 esterase ELISA Kit; C1S ELISA Kit; C1S_HUMAN ELISA Kit; Complement C1s subcomponent ELISA Kit; Complement C1s subcomponent heavy chain ELISA Kit; Complement C1s subcomponent light chain ELISA Kit; Complement component 1 s subcomponent ELISA Kit; Complement component 1 subcomponent s ELISA Kit; FLJ44757 ELISA Kit |
| Abbreviation |
C1S |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 ng/mL-1600 ng/mL |
| Sensitivity |
6.25 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
100 |
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| Range % |
91-104 |
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| 1:200 |
Average % |
107 |
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| Range % |
98-110 |
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| 1:400 |
Average % |
88 |
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| Range % |
82-93 |
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| 1:800 |
Average % |
96 |
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| Range % |
87-99 |
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| Recovery |
| The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
110 |
98-114 |
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| EDTA plasma (n=4) |
98 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1600 |
2.740 |
2.685 |
2.713 |
2.598 |
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| 800 |
2.346 |
2.276 |
2.311 |
2.196 |
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| 400 |
1.488 |
1.454 |
1.471 |
1.356 |
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| 200 |
0.756 |
0.768 |
0.762 |
0.647 |
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| 100 |
0.396 |
0.411 |
0.404 |
0.289 |
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| 50 |
0.246 |
0.239 |
0.243 |
0.128 |
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| 25 |
0.162 |
0.170 |
0.166 |
0.051 |
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| 0 |
0.113 |
0.117 |
0.115 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
