Human Complement factor H-related protein 1(CFHR1) ELISA kit
Product Details
| Target Name |
complement factor H-related 1 |
| Alternative Names |
CFHL ELISA Kit; CFHL1 ELISA Kit; CFHL1P ELISA Kit; CFHR1 ELISA Kit; CFHR1P ELISA Kit; Complement factor H related 1 ELISA Kit; Complement factor H related 1 pseudogene ELISA Kit; Complement factor H related protein 1 precursor ELISA Kit; Complement factor H-related protein 1 ELISA Kit; FHR 1 ELISA Kit; FHR-1 ELISA Kit; FHR1 ELISA Kit; FHR1_HUMAN ELISA Kit; H factor (complement) like 1 ELISA Kit; H factor (complement) like 2 ELISA Kit; H factor like protein 1 ELISA Kit; H factor-like protein 1 ELISA Kit; H-factor-like 1 ELISA Kit; H36 1 ELISA Kit; H36 2 ELISA Kit; H36 ELISA Kit; HFL1 ELISA Kit; HFL2 ELISA Kit; MGC104329 ELISA Kit |
| Abbreviation |
CFHR1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 ng/mL-1600 ng/mL |
| Sensitivity |
12.5 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
92-106 |
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| 1:200 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:400 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:800 |
Average % |
85 |
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| Range % |
80-89 |
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| Recovery |
| The recovery of human CFHR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-98 |
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| EDTA plasma (n=4) |
102 |
98-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 1600 |
0.089 |
0.090 |
0.090 |
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| 800 |
0.164 |
0.167 |
0.166 |
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| 400 |
0.279 |
0.288 |
0.284 |
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| 200 |
0.491 |
0.502 |
0.497 |
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| 100 |
0.754 |
0.740 |
0.747 |
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| 50 |
1.068 |
1.059 |
1.064 |
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| 25 |
1.545 |
1.447 |
1.496 |
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| 0 |
2.521 |
2.623 |
2.572 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
