Human connective tissue growth factor (CTGF) ELISA kit
Product Details
| Target Name |
connective tissue growth factor |
| Alternative Names |
CCN 2 ELISA Kit; CCN family member 2 ELISA Kit; CCN2 ELISA Kit; Connective tissue growth factor ELISA Kit; Ctgf ELISA Kit; CTGF_HUMAN ELISA Kit; Hcs 24 ELISA Kit; Hcs24 ELISA Kit; Hypertrophic chondrocyte specific protein 24 ELISA Kit; Hypertrophic chondrocyte-specific gene product 24 ELISA Kit; Hypertrophic chondrocyte-specific protein 24 ELISA Kit; IBP-8 ELISA Kit; IGF-binding protein 8 ELISA Kit; IGFBP-8 ELISA Kit; IGFBP8 ELISA Kit; Insulin like Growth Factor Binding Protein 8 ELISA Kit; Insulin-like growth factor-binding protein 8 ELISA Kit; MGC102839 ELISA Kit; NOV 2 ELISA Kit; NOV2 ELISA Kit |
| Abbreviation |
CTGF |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.75 pg/mL-120 pg/mL |
| Sensitivity |
1.875 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:2 |
Average % |
92 |
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| Range % |
86-96 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-96 |
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| 1:8 |
Average % |
86 |
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| Range % |
80-90 |
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| Recovery |
| The recovery of human CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
84-90 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 120 |
1.851 |
1.954 |
1.903 |
1.859 |
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| 60 |
1.078 |
1.087 |
1.083 |
1.039 |
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| 26.25 |
0.513 |
0.502 |
0.508 |
0.464 |
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| 11.25 |
0.255 |
0.268 |
0.262 |
0.218 |
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| 3.75 |
0.119 |
0.116 |
0.118 |
0.074 |
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| 0 |
0.043 |
0.044 |
0.044 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
