Product Details

Description

This human Cortisol (COR) ELISA kit employs the competitive inhibition enzyme immunoassay technique to quantitatively determine the human COR content in serum, plasma, cell culture supernates, saliva, and tissue homogenates, with a sensitivity of 0.049 ng/ mL. The kit has the advantages of high sensitivity, strong specificity, good repeatability, Intra-/inter-batch difference less than 10%, high recovery, and so on. Cortisol is the predominant physiological end-product of the hypothalamic-pituitary-adrenal (HPA) axis. Clinical studies found that elevated cortisol may exert toxic effects on cognition and contribute to the pathology of Alzheimer’s disease (AD). Standards or samples are added to the appropriate microtiter plate wells pre-coated with goat-anti-rabbit antibody. HRP-conjugated COR is pipped into the wells, where COR in the sample or standards competes with the HRP labeled COR for limited binding sites on the immobilized anti-COR antibody. The solution color turns blue after ...Read more

Abbreviation

COR

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.049 ng/mL-200 ng/mL

Sensitivity

0.049 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess.