Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit
Product Details
| Target Name |
Creatine Kinase MB isoenzyme,CK-MB |
| Alternative Names |
N/A |
| Abbreviation |
CK-MB |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.9 ng/mL-60 ng/mL |
| Sensitivity |
0.22 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:2 |
Average % |
104 |
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| Range % |
101-107 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
101 |
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| Range % |
98-104 |
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| Recovery |
| The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-89 |
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| EDTA plasma (n=4) |
109 |
104-113 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 60 |
2.016 |
2.106 |
2.061 |
1.907 |
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| 30 |
1.646 |
1.604 |
1.625 |
1.471 |
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| 15 |
1.215 |
1.253 |
1.234 |
1.080 |
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| 7.5 |
0.859 |
0.848 |
0.854 |
0.700 |
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| 3.75 |
0.586 |
0.552 |
0.569 |
0.415 |
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| 1.8 |
0.370 |
0.367 |
0.369 |
0.215 |
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| 0.9 |
0.284 |
0.278 |
0.281 |
0.127 |
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| 0 |
0.155 |
0.153 |
0.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
