Human CXC-chemokine receptor 4(CXCR4)ELISA Kit
Product Details
| Target Name |
chemokine (C-X-C motif) receptor 4 |
| Alternative Names |
C-X-C chemokine receptor type 4 ELISA Kit; CD184 ELISA Kit; CD184 antigen ELISA Kit; Chemokine (C X C motif) receptor 4 ELISA Kit; Chemokine CXC Motif Receptor 4 ELISA Kit; CXC-R4 ELISA Kit; CXCR-4 ELISA Kit; CXCR4 ELISA Kit; CXCR4_HUMAN ELISA Kit; D2S201E ELISA Kit; FB22 ELISA Kit; Fusin ELISA Kit; HM89 ELISA Kit; HSY3RR ELISA Kit; LAP 3 ELISA Kit; LAP3 ELISA Kit; LCR1 ELISA Kit; LESTR ELISA Kit; Leukocyte derived seven transmembrane domain receptor ELISA Kit; Leukocyte-derived seven transmembrane domain receptor ELISA Kit; Lipopolysaccharide associated protein 3 ELISA Kit; Neuropeptide Y receptor Y3 ELISA Kit; NPY3R ELISA Kit; NPYR ELISA Kit; NPYRL ELISA Kit; NPYY3 ELISA Kit; NPYY3R ELISA Kit; Probable G protein coupled receptor lcr1 homolog ELISA Kit; SDF 1 receptor ELISA Kit; SDF-1 receptor ELISA Kit; SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR ELISA Kit; Stromal cell derived factor 1 receptor ELISA Kit; Stromal cell-derived factor 1 receptor ELISA Kit; WHIM ELISA Kit; WHIMS ELISA Kit |
| Abbreviation |
CXCR4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CXCR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-102 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-93 |
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| Recovery |
| The recovery of human CXCR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
92-103 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.575 |
2.555 |
2.565 |
2.437 |
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| 500 |
1.928 |
2.074 |
2.001 |
1.873 |
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| 250 |
1.290 |
1.189 |
1.240 |
1.112 |
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| 125 |
0.612 |
0.603 |
0.608 |
0.480 |
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| 62.5 |
0.394 |
0.382 |
0.388 |
0.260 |
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| 31.2 |
0.256 |
0.245 |
0.251 |
0.123 |
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| 15.6 |
0.201 |
0.199 |
0.200 |
0.072 |
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| 0 |
0.133 |
0.123 |
0.128 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
