Human cyclooxygenase-2,COX-2 ELISA Kit
Product Details
| Target Name |
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
| Alternative Names |
COX 2 ELISA Kit; COX-2 ELISA Kit; COX2 ELISA Kit; Cyclooxygenase 2 ELISA Kit; Cyclooxygenase 2b ELISA Kit; Cyclooxygenase ELISA Kit; Cyclooxygenase-2 ELISA Kit; Cyclooxygenase2 ELISA Kit; EC 1.14.99.1 ELISA Kit; fj02a10 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase ELISA Kit; GRIPGHS ELISA Kit; hCox 2 ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; OTTHUMP00000033524 ELISA Kit; PES-2 ELISA Kit; PGG/HS ELISA Kit; PGH synthase 2 ELISA Kit; PGH2_HUMAN ELISA Kit; PGHS 2 ELISA Kit; PGHS-2 ELISA Kit; PGHS2 ELISA Kit; PHS 2 ELISA Kit; PHS II ELISA Kit; PHS2 ELISA Kit; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin endoperoxide synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 precursor ELISA Kit; Prostaglandin G/H synthase and cyclooxygenase ELISA Kit; Prostaglandin G/H synthase ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; PTGS2 ELISA Kit; ptgs2a ELISA Kit; TIS10 ELISA Kit; TIS10 protein ELISA Kit; unp1239 ELISA Kit; wu:fj02a10 ELISA Kit |
| Abbreviation |
PTGS2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
1.25 ng/mL-80 ng/mL |
| Sensitivity |
0.31 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
84 |
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| Range % |
80-88 |
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| Recovery |
| The recovery of human COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
87-100 |
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| EDTA plasma (n=4) |
88 |
82-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.701 |
2.652 |
2.677 |
2.583 |
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| 40 |
2.092 |
1.949 |
2.021 |
1.927 |
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| 20 |
1.301 |
1.228 |
1.265 |
1.171 |
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| 10 |
0.587 |
0.571 |
0.579 |
0.485 |
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| 5 |
0.357 |
0.374 |
0.366 |
0.272 |
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| 2.5 |
0.207 |
0.213 |
0.210 |
0.116 |
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| 1.25 |
0.152 |
0.161 |
0.157 |
0.063 |
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| 0 |
0.092 |
0.096 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
