Human Cystatin-B (CSTB/CST6/STFB) ELISA kit
Product Details
| Target Name |
cystatin B (stefin B) |
| Alternative Names |
CHROW21 ELISA Kit; CPI B ELISA Kit; CPI-B ELISA Kit; CST 6 ELISA Kit; CST6 ELISA Kit; CSTB ELISA Kit; Cystatin B (stefin B) ELISA Kit; Cystatin B ELISA Kit; Cystatin-B ELISA Kit; CYTB ELISA Kit; CYTB_HUMAN ELISA Kit; EPM1 ELISA Kit; EPM1A ELISA Kit; Liver thiol proteinase inhibitor ELISA Kit; PME ELISA Kit; Stefin-B ELISA Kit; STF B ELISA Kit; STFB ELISA Kit; ULD ELISA Kit |
| Abbreviation |
CSTB |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine, saliva, tissue homogenates, cell lysates |
| Detection Range |
0.313 ng/mL-20 ng/mL |
| Sensitivity |
0.233 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CSTB/CST6/STFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
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| Range % |
101-106 |
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| 1:2 |
Average % |
91 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-104 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-94 |
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| Recovery |
| The recovery of human CSTB/CST6/STFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
99-108 |
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| EDTA plasma (n=4) |
98 |
93-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.411 |
2.588 |
2.500 |
2.340 |
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| 10 |
1.702 |
1.811 |
1.757 |
1.597 |
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| 5 |
1.166 |
1.163 |
1.165 |
1.005 |
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| 2.5 |
0.654 |
0.699 |
0.677 |
0.517 |
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| 1.25 |
0.423 |
0.411 |
0.417 |
0.257 |
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| 0.625 |
0.266 |
0.255 |
0.261 |
0.101 |
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| 0.313 |
0.203 |
0.211 |
0.207 |
0.047 |
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| 0 |
0.155 |
0.164 |
0.160 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
