Human Cytochrome-C,Cyt-C ELISA Kit
Product Details
| Target Name |
cytochrome c, somatic |
| Alternative Names |
CYC ELISA Kit; CYC_HUMAN ELISA Kit; CYCS ELISA Kit; Cytochrome c ELISA Kit; Cytochrome c somatic ELISA Kit; HCS ELISA Kit; THC4 ELISA Kit |
| Abbreviation |
CYCS |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell lysates |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
82-105 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-107 |
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| 1:8 |
Average % |
90 |
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| Range % |
83-98 |
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| Recovery |
| The recovery of human Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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| EDTA plasma (n=4) |
95 |
88-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
2.337 |
2.404 |
2.371 |
2.222 |
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| 250 |
1.692 |
1.659 |
1.676 |
1.527 |
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| 125 |
1.106 |
1.138 |
1.122 |
0.973 |
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| 62.5 |
0.675 |
0.662 |
0.669 |
0.520 |
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| 31.2 |
0.436 |
0.428 |
0.432 |
0.283 |
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| 15.6 |
0.257 |
0.273 |
0.265 |
0.116 |
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| 7.8 |
0.206 |
0.215 |
0.211 |
0.062 |
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| 0 |
0.153 |
0.145 |
0.149 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
