Human Cytochrome P450 19A1(CYP19A1) ELISA kit
Product Details
| Target Name |
cytochrome P450, family 19, subfamily A, polypeptide 1 |
| Alternative Names |
ARO ELISA Kit; ARO1 ELISA Kit; Aromatase ELISA Kit; CP19A_HUMAN ELISA Kit; CPV1 ELISA Kit; CYAR ELISA Kit; CYP19 ELISA Kit; Cyp19a1 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Cytochrome P450; family 19; subfamily A; polypeptide 1 ELISA Kit; Cytochrome P450; subfamily XIX (aromatization of androgens) ELISA Kit; Estrogen synthase ELISA Kit; Estrogen synthetase ELISA Kit; Flavoprotein linked monooxygenase ELISA Kit; MGC104309 ELISA Kit; Microsomal monooxygenase ELISA Kit; OTTHUMP00000162543 ELISA Kit; OTTHUMP00000198350 ELISA Kit; P 450AROM ELISA Kit |
| Abbreviation |
CYP19A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
26.7 pg/mL-1000 pg/mL |
| Sensitivity |
6.7 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:4 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:8 |
Average % |
85 |
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| Range % |
80-89 |
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| Recovery |
| The recovery of human CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
91 |
86-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.238 |
2.217 |
2.228 |
2.192 |
|
| 533.3 |
1.665 |
1.612 |
1.639 |
1.603 |
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| 266.7 |
1.034 |
1.037 |
1.036 |
1.000 |
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| 66.7 |
0.309 |
0.301 |
0.305 |
0.269 |
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| 26.7 |
0.157 |
0.161 |
0.159 |
0.123 |
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| 0 |
0.037 |
0.034 |
0.036 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
