Human Cytokeratin 18-M30,CK 18-M30 ELISA kit
Product Details
| Target Name |
Cytokeratin 18-M30,CK 18-M30 |
| Alternative Names |
N/A |
| Abbreviation |
CK 18M30 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
15.6 mIU/mL-1000 mIU/mL |
| Sensitivity |
3.9 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
86 |
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| Range % |
81-92 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:8 |
Average % |
89 |
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| Range % |
83-96 |
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| Recovery |
| The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
99 |
94-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.577 |
2.552 |
2.565 |
2.443 |
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| 500 |
2.056 |
2.016 |
2.036 |
1.914 |
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| 250 |
1.267 |
1.202 |
1.235 |
1.113 |
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| 125 |
0.683 |
0.648 |
0.666 |
0.544 |
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| 62.5 |
0.379 |
0.405 |
0.392 |
0.270 |
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| 31.2 |
0.294 |
0.279 |
0.287 |
0.165 |
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| 15.6 |
0.201 |
0.199 |
0.200 |
0.078 |
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| 0 |
0.117 |
0.126 |
0.122 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
