Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit
Product Details
| Target Name |
cytokeratin fragment antigen 21-1,CYFRA21-1 |
| Abbreviation |
CYFRA21-1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-90 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-110 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-99 |
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| Recovery |
| The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
80-98 |
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| EDTA plasma (n=4) |
97 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.541 |
2.385 |
2.463 |
2.305 |
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| 10 |
2.229 |
2.082 |
2.156 |
1.998 |
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| 5 |
1.607 |
1.545 |
1.576 |
1.418 |
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| 2.5 |
0.866 |
0.842 |
0.854 |
0.696 |
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| 1.25 |
0.463 |
0.439 |
0.451 |
0.293 |
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| 0.625 |
0.337 |
0.324 |
0.331 |
0.173 |
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| 0.312 |
0.254 |
0.246 |
0.250 |
0.092 |
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| 0 |
0.160 |
0.156 |
0.158 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
