Human D-Lactate Dehydrogenase,D-LDH ELISA Kit
Product Details
| Target Name |
lactate dehydrogenase D |
| Alternative Names |
D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit |
| Abbreviation |
LDHD |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 mIU/mL-2000 mIU/mL |
| Sensitivity |
7.8 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
87-103 |
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| 1:2 |
Average % |
91 |
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| Range % |
86-103 |
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| 1:4 |
Average % |
94 |
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| Range % |
86-105 |
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| 1:8 |
Average % |
91 |
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| Range % |
84-100 |
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| Recovery |
| The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
91-106 |
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| EDTA plasma (n=4) |
96 |
86-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.418 |
2.385 |
2.402 |
2.265 |
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| 1000 |
1.773 |
1.728 |
1.751 |
1.614 |
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| 500 |
0.936 |
1.002 |
0.969 |
0.832 |
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| 250 |
0.624 |
0.651 |
0.638 |
0.501 |
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| 125 |
0.407 |
0.422 |
0.415 |
0.278 |
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| 62.5 |
0.282 |
0.287 |
0.285 |
0.148 |
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| 31.25 |
0.253 |
0.264 |
0.259 |
0.122 |
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| 0 |
0.135 |
0.139 |
0.137 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
